Coding

Part:BBa_K2926048:Design

Designed by: Johanna Opgenoorth   Group: iGEM19_Bielefeld-CeBiTec   (2019-10-15)


mCherry with hexahistidine tag for purification


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To allow a simplified purification we designed a coding sequence for a hexahistidine tag that was inserted between the last codon of mCherry and the stop-codon. We used two different codons for histidine to improve the translation.


Source

Modified from Part BBa_J06504

References